International Conference on Future Trends In Bio-Informatics and Environmental Science

Proceedings of

International Conference on Future Trends In Bio-Informatics and Environmental Science FTBES 2014

Date

05-May-2014

Location

Bangkok , Thailand

Authors

11

ISBN

978-1-63248-016-3

5 Articles Published

1. ANALYSIS OF ANTIMITOTIC ACTIVITIES FROM PHYTONUTRIENTS

Authors: JILSA.JAISON , POOJA.KP , PRIYANKA.RAVICHANDRAN

Abstract: Ayurveda, an ancient form of Indian medicine, speaks of several plant parts that cure different ailments when consumed. Here, we analyze different regularly consumed plant products to study their effect on mitosis. Several extracts were able to suppress mitosis, Areca nut was picked as a known mutagen, and turmeric and mango for their antimitotic activity upon eliminating other plant extracts. Preliminary analysis of a mixture of the mitogenic and antimitotic extracts showed that the antimitotic extracts were able to suppress the mitogenic activity of areca nut, indicating that the activity of areca nut is one of the factors that may be dependent on those targeted by mango and turmeric. Extracts prepared in absolute ethanol showed that the active ingredient is either inactive or absent in the ethanol extract. Two approaches were taken to identify the active ingredient. Firstly, the extracts were tested for the presence of known active ingredients, such as tannins, that are known to hav

Keywords: Analysis, antimitotic, activities, phytonutrients

Pages: 1 - 5 | DOI: 10.15224/978-1-63248-016-3-01

2. FABRICATION AND MECHANICAL CHARACTERIZATION OF THAI SILK FIBROIN NANOFIBROUS SCAFFOLDS

Authors: SATHIT BANTHUEK , SOMCHAI PATTANA

Abstract: Silk fibroin from Thai Bombyx mori (Nangnoi Srisaket-I) cocoons was interested to use as biomaterial for tissue engineering scaffolds. In this paper, Thai silk fibroin based nanofibrous scaffolds were fabricated by electrospinning technique. The effects of electrospinning parameters, such as silk fibroin/formic acid solutions concentrations and applied voltage, on the morphology and mechanical properties of Thai silk fibroin based nanofibrous scaffold were investigated. Thai Silk fibroin (SF) sponges were dissolved in 98% formic acid with concentration of 20, 30, and 40 % (w/v) and spun into fibers at applied voltage of 15, 20, and 25 kV, respectively. The morphological and mechanical properties of SF-nanofibers were examined by field emission scanning electron microscopy (FESEM) and AFM indentation method. Electrospinning technique could produce nanofibers in the range of diameter from 100–300 nm. The concentration of silk fibroin solution was considered as the most important paramete

Keywords: silk fibroin, nanofibrous scaffold, electrospinning, mechanical properties, tissue engineering

Pages: 6 - 10 | DOI: 10.15224/978-1-63248-016-3-02

3. DESIGN OF SENSOR CHIP TO REPEL THE NON-SPECIFIC BINDING FOR ITS APPLICATION IN BIOMEDICAL SENSOR; EFFECT OF HUMAN SERUM SAMPLES

Authors: THIDARAT WANGKAM

Abstract: In biomedical application, it is essential to perform the detection in human serum in most of cases. However, the main difficulty of using serum is high non-specific binding between the sensor surface and serum proteins. The modifications of sensor surfaces were studied in this work. The self assemble monolayer (SAMs) and hydrophilic polymer dextran were coated on gold substrate with chemisorption method and was tested in the property of a repelling the non-specific binding of human serum by monitoring via Surface Plasmon resonance technique (SPR) gtecnique. SPR is an optical technique, which is highly sensitive on the change of the optical properties of the biomolecules in nanoscale. The results were shown that there was effect of serum dilutions on both of the sensors. It was shown the effect of increasing the human serum concentration on the SPR signal increasing. The efficiency of repelling human serum in both SAMs and dextran was shown different. Dextran gave a lower non-specific

Keywords: Surface Plasmon resonance, thin film, nonspecific binding

Pages: 11 - 14 | DOI: 10.15224/978-1-63248-016-3-03

4. RESISTANCE AND PLANT GROWTH-PROMOTING PROPERTIES UNDER ZN/CD STRESS OF PSEUDOMONAS SP. ZNCD2003

Authors: APHIDECH SANGDEE , PAYUNGSAK TABOONMA, , PAYUNGSAK TABOONMA

Abstract: Pseudomonas sp. PDMZnCd2003 is a plant growthpromoting bacteria (PGPB) that was isolated from Zn/Cd contaminated soil. This research aims to study the characteristics of the Zn/Cd resistance and plant growth promoting properties of Pseudomonas sp.PDMZnCd2003 under Zn/Cd stress. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods were carried out to evaluate the lowest concentrations of Zn and/or Cd that inhibited and killed the bacterium. The MIC concentrations for Zn, Cd, Zn plus Cd and fixed Cd plus Zn were 150 mg l-1, 70 mg l-1, 60/60 mg l-1 and 20/150 mg l-1, respectively. The MBC concentrations for Cd were 300 mg l-1, while the bacterial growth recovered from treatment with 400 mg l-1 of Zn, 100/100 mg l-1 of Zn plus Cd, and 20/2000 mg l-1 of fixed Cd plus Zn. The growth tended to decrease under Zn/Cd stress, however, the plant growth properties of IAA production, N2 fixation and P solubilisation remained under Zn plus Cd at 20/20 mg l-1. Du

Keywords: Pseudomonas, zinc, cadmium, plant growth promoting bacteria

Pages: 15 - 20 | DOI: 10.15224/978-1-63248-016-3-04

5. METEOROLOGICAL EFFECTS ON GROUND-LEVELS OZONE METRICS IN BANGKOK METROPOLIS REGION

Authors: BUNDIT APISAMAJARAKUL , SITTHICHOK PUANGTHONGTHUB

Abstract: Multiple linear regression models were constructed to characterize ground-level O3 metrics in Bangkok Metropolis Region where meteorological parameters are different from other studies in cold cities. SAS® 9.2 software analyzed 2.9-million hourly data during 1997 – 2011 including O3, NO2 and meteorological variables such as temperature (T), rainfall (RF), relative humidity (RH), pressure (P), solar radiation (SR), wind speed (WS) and wind direction (WD). The results showed O3 was highest in winter because of clearest sky and an atmospheric inversion. O3 had negatively correlated with RH and RF and positively correlated with SR and previous day O3 (O3(d-1)) Natural logarithm transformed O3 was used for 3 O3 metrics (daily average, daily maximum and daytime average) for 4 periods (annual, summer, winter and rainy season). Regression results showed that the lnO3(d-1) was a main positive predictor and RH is the strongest negative predictor following by a positive SR predictor. In winter, m

Keywords: ozone, meteorology, multiple linear regression, seasonal effects

Pages: 21 - 25 | DOI: 10.15224/978-1-63248-016-3-05