Abstract: “Modeling the regulation of lactose operon expression is significant to understand lactose metabolism at the cell-level, in which the repressor and cAMP-CRP can influence its regulation process. In this paper, a mathematic model of lactose operon expression is established in differential equations with the knowledge of system biology. Considering multiple factors such as lactose, glucose, repressor, permease, β-galactosidase, allolactose and cAMP-CRP, this paper presents dynamic bi-direction regulatory mechanisms in both repressor and cAMP-CRP with the logical “AND” of system biology. The experimental data indicates that the proposed model is more approach to the real condition.”

Keywords: system biology, mathematical modeling, lactose operon expression