Abstract: “The disruption of genetic materials as the aberrant methylation at CpG islands of tumor suppressor gene’s promoter, which is thought to be a driver epigenetic events of malignant cells. p16INK4α, its function is considered as an inhibitor of CDK4 and CDK6 that initiate the phosphorylation of RB (retinoblastoma tumor suppressor protein), has been identified as a tumor suppressor gene in many human cancers. In current study, methylation specific PCR (MSP) was carried out to evaluate the methylation status of p16INK4α from Vietnamese breast patients, in order to find out the methylation signature served as biomarker applied in prognosis, early detection and diagnosis of breast cancer in Vietnamese population. The results showed that the methylation frequency of p16INK4α promoter reached to 49.5% (47 of 95 breast cancer samples). In the case of non-cancer specimens, only 15% (3 of 20 non-cancer specimens) was methylation. According to clinical parameters, there were significant correlation”

Keywords: breast cancer, methylation, MSP, p16INK4α , Vietnamese breast cancer.