Abstract: “Protecting our environment, while stabilizing the population and adequately feeding the people who will share the Earth in the next generation, is the largest challenge facing human kind today. The preservation of biodiversity can be accomplished only as part of an overall strategy to promote global stability. India is one of the richest countries in the world in terms of biodiversity. It is known worldwide for its rich repository of spices and thus popularly known as the "land of spices". Among various spices, seed spices play a major role in Indian history and commerce as they are cultivated in India since ancient times; also India enjoys the position of being the largest producer, consumer and exporter of seed spices in the world. Cuminum cyminum L. is an important seed spice belonging to family Apiaceae. Production of cumin is limited due to several biotic stresses of which wilt diseases are the most serious. Efforts to improve cumin are limited due to the unavailability of broad g”

Keywords: Cumin, Calcium, Regeneration, peroxidase, proliferation.