Abstract: “Lactic acid is one of the functional and valuable compounds utilized in food, pharmaceutical, and chemical industries while Poly lactic acid (PLA) is a biodegradable polymer that has a variety of applications. In recent years, microbial conversion of renewable raw materials has become an important objective in industrial biotechnology. Among many raw materials available, tapioca starch is considered an interesting resource for lactic acid fermentation as it is costeffective and its availability is satisfactory. After being hydrolyzed, a hydrolyte of cassava (tapioca starch) containing high concentrations of glucose and a few other fermentable sugars was obtained. This is feasible for an efficient lactic acid production. The objective of this study was to screen and analyze important nutrient constituents was carried out using Plackett- Burman experimental design for production of lactic acid by Lactobacillus rhamnosus B14-2 grown in cassava fermentation. Plackett-Burman experimental de”

Keywords: Lactic acid, lactobacillus rhamnosus B14-2, cassava, poly lactic acid, renewable raw material