Abstract: “Due to stringent environment regulations, the disposal of cotton gin waste is one of the massive problems faced by cotton industries throughout the world. Cotton gin waste is a lignocellulosic biomass and this might be utilized to produce bioethanol which is a promising alternative energy sources for transportation fuel. The prime effort of the present investigation was to decompose or degrade the complex mixtures of cellulose, hemicellulose and lignin compounds of cotton gin waste. This pretreatment has enhanced the production of reducing sugar and bio-ethanol using mixed culture of efficient white rot fungi (Trametes pubscens and Pycnoporus cinnabarinus).It was examined that, in delignification process, solid state cultivation (SSC) having 57.5 % of lignin removal was found to be more effective than submerged cultivation (SMC) with 45.6 % of lignin removal.The corresponding cellulose and hemicellulose reduction were determined as 64 and 68.5% in SSC, whereas their values in SMC were”

Keywords: Cotton gin waste, mixed culture, white rots fungi, lignin, cellulose, hemicelluloses